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The first drug made using recombinant DNA technology was human insulin (Humulin), produced in E. coli.
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It is used for the treatment of diabetes mellitus.
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Recombinant DNA avoided the earlier problem of allergic reactions to animal insulin.
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What is the term for the ability of a plant cell to regenerate into a whole plant?
What is micro-propagation in plant tissue culture?
Plants produced by tissue culture that are genetically identical to the original plant are called:
Which plant tissue is primarily used to obtain disease-free plants, even from virus-infected stock?
Removing the cell wall to isolate naked protoplasts enables what process?
A fusion between protoplasts from two different plant species to generate a hybrid is called:
The novel plant ‘pomato‘ (a potato–tomato hybrid) was produced by:
Somatic hybridisation is particularly useful for:
Which step follows protoplast fusion in somatic hybridisation?
Which of the following is not an advantage mentioned for somaclonal variations?
Somaclonal variations are:
The capacity of a plant cell or explant to regenerate a complete plant is termed:
The rapid multiplication of identical plants under controlled conditions in nutrient medium is known as:
Protoplasts are generated by enzymatic digestion of:
Somatic hybridization is especially useful to incorporate traits from species that:
(c)
Corn borer can be controlled by the proteins encoded by Bacillus thuringiensis gene :
(a)
RNAi, as a mechanism of cellular defense, takes place in :
(d)
Nematode [Meloidogyne incognita] specific genes were transferred to tobacco plant using :
Antisense technology :
(a)
The first drug produced using recombinant DNA technology is used to treat :
(d)
The first drug made using recombinant DNA technology was human insulin (Humulin), produced in E. coli.
It is used for the treatment of diabetes mellitus.
Recombinant DNA avoided the earlier problem of allergic reactions to animal insulin.
The two polypeptide chains of insulin protein are linked together by :
(a)
Which of these is an accurate description of gene therapy ?
(b)
Gene therapy for ADA deficiency has been tried with the help of which of the following vectors ?
(d)
A possible permanent cure for SCID can be :
(d)
ADA is an enzyme which is deficient in a genetic disorder SCID. What is the full form of ADA ?
(b)
A probe which is a molecule used to locate homologous sequences in a mixture of DNA or RNA molecules could be :
(c)
Silencing of a gene could be achieved through the use of :
(c)
ELISA is based on the principle of :
(b)
Alpha-1-antitrypsin was produced in a transgenic sheep, Tracy. This enzyme is used in the treatment of :
(a)
At present transgenic animals cannot be used for :
(d)
1. Harvesting biological products → Possible (e.g., transgenic goats producing antithrombin in milk).
2. Testing vaccine safety → Possible (e.g., transgenic mice used for polio vaccine testing).
3. Study of human diseases → Possible (e.g., transgenic models for cystic fibrosis, cancer).
4. Xenotransplantation → Not yet feasible in practical human medicine due to strong immune rejection and ethical issues; currently experimental only.
Transgenic mice are being used to test the safety of polio vaccine. They could replace the use of :
(a)
Bt toxin kills pest by :
(c)
Which of the following genes was integrated in pBR322 based vector for humulin synthesis ?
(d)
Which of the following is incorrect for Flavr-Savr ?
(d)
Statement 4: “It was produced for more taste and less seed” ❌ Incorrect
NCERT mentions delayed ripening, not enhanced taste or fewer seeds.
Humulin was produced by :
(c)
Severe Combined Immunodeficiency Disorder is caused due to deletion of gene for which of the following enzymes?
(d)
Cotton bollworm can be controlled by :
(b)
Which of the following statements is correct with respect to RNAi ?
(a)
The invertebrate which is a causative agent of the root knot disease in tobacco plant is
(a)
One of the key factors, which makes the plasmid the vector in genetic engineering in that-
(c)
RNAi is a mechanism to silence genes with the help of :
(b)
The genetically engineered vaccine for the prevention of hepatitis B is made with the help of the microbe :
(c)
The first clinical gene therapy was done for the treatment of :
(d)
Why does Bt toxin not kill Bacillus thuringiensis ?
(b)
Exploring molecular, genetic, and species-level diversity for products of economic importance unethically appropriated and without fair compensation to indigenous people is called:
(c)
Cotton plants can be protected from corn borer by the proteins coded by :
(c)
Consider the two statements:
\(
\begin{array}{|l|l|}
\hline \text { I: } & \text { Retroviruses are useful as vectors in gene therapy. } \\
\hline \text { II: } & \begin{array}{l}
\text { They can produce useful gene products in } \\
\text { significantly greater quantities than a single human } \\
\text { cell can. }
\end{array} \\
\hline
\end{array}
\)
(c)
Statement II: “They can produce useful gene products in significantly greater quantities than a single human cell can.” ❌ Incorrect
Retroviruses themselves are not used to produce large quantities of proteins. That role is for bacterial or yeast expression systems. Retroviruses are used mainly as delivery vectors, not for mass protein production.
How were nematode-specific genes introduced in tobacco plants ?
(d)
A fragment of nucleic acid (DNA or RNA) that can be used to identify, usually through autoradiography, specific DNA or RNA molecules bearing the complementary sequence is called:
ELISA is based on the principle :
(b)
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