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The figure shows a polynucleotide chain.
\(
\begin{array}{|l|l|}
\hline \begin{array}{l}
\text { Statement } \\
\text { I: }
\end{array} & \begin{array}{l}
\text { A is the 5′ end of the polynucleotide chain and } \\
\text { B is the 3′ end of the polynucleotide chain. }
\end{array} \\
\hline \begin{array}{l}
\text { Statement } \\
\text { II: }
\end{array} & \begin{array}{l}
\text { The nitrogenous bases linked to sugar moiety } \\
\text { project from the backbone. }
\end{array} \\
\hline
\end{array}
\)
(d)
Consider the following Statements :-
\(
\begin{array}{|l|l|}
\hline \text { I: } & \begin{array}{l}
\text { DNA – deoxyribonucleic acid – is the genetic } \\
\text { material, at least for the majority of organisms. }
\end{array} \\
\hline \text { II: } & \begin{array}{l}
\text { RNA though it also acts as a genetic material in some } \\
\text { viruses, mostly functions as a messenger. }
\end{array} \\
\hline \text { III. } & \begin{array}{l}
\text { RNA can function as an adapter, structural, and in } \\
\text { some cases as a catalytic molecule. }
\end{array} \\
\hline
\end{array}
\)
(a)
Study the structure of double-stranded nucleotide chain given below and select the correct statements :
\(
\begin{array}{|l|l|}
\hline \begin{array}{l}
\text { Statement } \\
\text { I: }
\end{array} & \begin{array}{l}
\text { A shows the hydrogen bonds where a triple } \\
\text { bond links Guanine and Cytosine and a double } \\
\text { bond links Adenine and Thymine. }
\end{array} \\
\hline \begin{array}{l}
\text { Statement } \\
\text { II: }
\end{array} & \begin{array}{l}
\text { If Adenine in such a dsDNA molecule is } \\
\text { found to be } 20 \%, \text { the amount of Cytosine in } \\
\text { this ds DNA molecule is expected to be } 20 \% .
\end{array} \\
\hline
\end{array}
\)
(c)
I: Strands are antiparallel | ❌ Incorrect | Diagram shows both strands as parallel (5′ → 3′ same direction) |
II: Base pairing follows Chargaff’s rule | ❌ Incorrect | Base pairs shown on same strand polarity (e.g., G≡C both on 5′ ends), which violates actual base pairing rules |
To form a nucleoside, a nitrogenous base is linked to a pentose sugar :
\(
\begin{array}{|c|l|}
\hline \text { 1. } & \begin{array}{l}
\text { Through a P-Glycosidic linkage at carbon atom } \\
\text { number 1 }
\end{array} \\
\hline \text { 2. } & \begin{array}{l}
\text { Through a P-Glycosidic linkage at carbon atom } \\
\text { number 5 }
\end{array} \\
\hline \text { 3. } & \begin{array}{l}
\text { Through an N-Glycosidic linkage at carbon atom } \\
\text { number 1 }
\end{array} \\
\hline \text { 4. } & \begin{array}{l}
\text { Through an N-Glycosidic linkage at carbon atom } \\
\text { number 5 }
\end{array} \\
\hline
\end{array}
\)
(c)
The diagram shows the DNA double helix. What will be true for the helix ?
\(
\begin{array}{|l|l|}
\hline \text { I. } & \begin{array}{l}
\text { A represents a base pair where the plane of one base } \\
\text { pair stacks over the other in the double helix giving } \\
\text { the helix more stability in addition to hydrogen } \\
\text { bonds. }
\end{array} \\
\hline \text { II. } & \begin{array}{l}
\text { There are } 10 \text { base pairs in one complete turn of B- } \\
\text { dNA. }
\end{array} \\
\hline \text { III. } & \text { B is the backbone constituted by sugar-phosphate. } \\
\hline \text { IV. } & \begin{array}{l}
\text { The two strands are parallel-oriented in the same } \\
\text { direction with respect to each other. }
\end{array} \\
\hline \text { V: } & \text { The diameter is uniform, around } 2.0 \mathrm{~nm} . \\
\hline
\end{array}
\)
(c)
IV : The two stands are anti parallel not parallel
According to Erwin Chargaff, in a double-stranded DNA :-
(a)
The central dogma of molecular biology is an explanation of the flow of genetic information within a biological system :
Statement I: A is ‘replication‘ where DNA is replicated in a semi-conservative manner.
Statement II: B is ‘transcription‘ and is so called because the language of RNA is only a slight variant of the language of the DNA.
Statement III: C is ‘translation‘ and is so called because the language of Protein is entirely different from the language of RNA.
(c)
All correct
The structure of nucleosome is shown below :
I: A is H1 histone.
II: B is a tetramer made of histone proteins.
The given figure shows the Electron Microscope ‘beads-on-string’ structure of :
(a)
Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
\(
\begin{array}{|l|l|}
\hline \text { Assertion (A): } & \begin{array}{l}
\text { The distance between the two strands of } \\
\text { the DNA helix is approximately uniform. }
\end{array} \\
\hline \text { Reason (R): } & \begin{array}{l}
\text { base pairing is complementary where a } \\
\text { purine always base pairs with a } \\
\text { pyrimidine and a pyrimidine always base } \\
\text { pairs with a purine. }
\end{array} \\
\hline
\end{array}
\)
In the light of the above statements, choose the most appropriate answer from the options given below :
(a)
Consider the two statements:
\(
\begin{array}{|l|l|}
\hline \text { I: } & \begin{array}{l}
\text { If the sequence of bases in one strand of a double- } \\
\text { stranded DNA is known, then the sequence in other } \\
\text { strands can be predicted. }
\end{array} \\
\hline \text { II: } & \begin{array}{l}
\text { If each strand from a DNA acts as a template for the } \\
\text { synthesis of a new strand, the two double-stranded } \\
\text { DNA thus, produced would be identical to the } \\
\text { parental DNA molecule. }
\end{array} \\
\hline
\end{array}
\)
(c)
Histones are rich in amino acids :
(a)
Study the illustration of the basic steps of the Hershey–Chase experiment and select the correct statements from the given experiments :
I: ‘A‘ shows that viruses grown on radioactive sulfur contained radioactive protein but not radioactive DNA because DNA does not contain sulfur.
II. ‘B‘ shows that viruses are grown in the presence of radioactive phosphorus contained radioactive DNA but not radioactive protein because DNA contains phosphorus but protein does not.
III: The result ‘X‘ shows bacteria that were infected with viruses that had radioactive DNA were radioactive, indicating that DNA was the material.
IV. The result ‘Y‘ shows bacteria that were infected with viruses that had radioactive proteins were not radioactive indicating that proteins did not enter the bacteria from the viruses.
(a)
Statement III:- ❌ Incorrect – ‘X’ is the left panel (‘A’), which used radioactive sulfur (protein), and the bacteria did not become radioactive.
Statement IV:- ❌ Incorrect – this actually mixes up the panels:
Given below are two statements : one is labelled as
Assertion (A) and the other is labelled as Reason (R).
\(
\begin{array}{|l|l|}
\hline \text { Assertion (A): } & \begin{array}{l}
\text { A set of positively charged and basic } \\
\text { proteins are used in the packaging of } \\
\text { Eukaryotic DNA. }
\end{array} \\
\hline \text { Reason (R): } & \begin{array}{l}
\text { DNA molecule is acid and carries a } \\
\text { negative charge. }
\end{array} \\
\hline
\end{array}
\)
In the light of the above statements, choose the most appropriate answer from the options given below :
(a)
During DNA packaging in eukaryotic cells, Non-Histone Chromosomal proteins :
(d)
Given below are two statements : one is labelled as
Assertion (A) and the other is labelled as Reason (R).
\(
\begin{array}{|l|l|}
\hline \text { Assertion (A): } & \begin{array}{l}
\text { Euchromatin is said to transcriptionally } \\
\text { active protein. }
\end{array} \\
\hline \text { Reason (R): } & \begin{array}{l}
\text { Heterochromatin is said to be } \\
\text { transcriptionally inactive. }
\end{array} \\
\hline
\end{array}
\)
In the light of the above statements, choose the most appropriate answer from the options given below :
(b)
Saying heterochromatin is inactive doesn’t explain why euchromatin is active.
It just provides a contrasting fact, not a causal reason.
An actual explanation of (A) would involve stating that loose packing in euchromatin allows easy access to transcription machinery.
The given Fig. Shows :-
(a)
In Griffith’s experiment, which of the following bacterial strains was capable of causing pneumonia in mice ?
(b)
Given below are two statements : one is labelled as
Assertion (A) and the other is labelled as Reason (R).
\(
\begin{array}{|l|l|}
\hline \text { Assertion (A): } & \begin{array}{l}
\text { S-strain of Streptococcus pneumoniae is } \\
\text { virulent. }
\end{array} \\
\hline \text { Reason (R): } & \begin{array}{l}
\text { Heat-killed S-strain Streptococcus } \\
\text { pneumoniae injected into mice did not } \\
\text { cause pneumonia and did not kill them. }
\end{array} \\
\hline
\end{array}
\)
In the light of the above statements, choose the most appropriate answer from the options given below :
(c)
Assertion (A) is stating why the live S-strain is virulent (due to its capsule).
But Reason (R) is about the heat-killed S-strain, which is no longer virulent.
So, R is not giving the reason for A’s virulence, it’s just another observation from the experiment.
Given below are two statements : one is labelled as
Assertion (A) and the other is labelled as Reason (R).
\(
\begin{array}{|l|l|}
\hline \text { Assertion (A): } & \begin{array}{l}
\text { In Avery, Macleod, and McCarty’s } \\
\text { experiment, digestion of heat-killed } \\
\text { lysate with proteases and RNases did not } \\
\text { affect transformation but digestion with } \\
\text { DNase did. }
\end{array} \\
\hline \text { Reason (R): } & \begin{array}{l}
\text { DNase will hydrolyse all biomolecules in } \\
\text { the lysate except DNA. }
\end{array} \\
\hline
\end{array}
\)
In the light of the above statements, choose the most appropriate answer from the options given below :
(a)
The figure shows the Meselson and Stahl experiment. Identify the correct statements :
I: The result at the end of 20 minutes excludes the ‘conservative’ mode of DNA replication.
II: The result at the end of 40 minutes excludes the ‘dispersive’ mode of DNA replication.
(d)
Given below are two statements : one is labelled as
Assertion (A) and the other is labelled as Reason (R)
\(
\begin{array}{|l|l|}
\hline \text { Assertion (A): } & \begin{array}{l}
\text { Even after the results of Avery, Macleod, } \\
\text { and McCarty’s experiment, not all } \\
\text { biologists were convinced that DNA is } \\
\text { hereditary material. }
\end{array} \\
\hline \text { Reason (R): } & \begin{array}{l}
\text { Proteins were conclusively proved to be } \\
\text { the genetic material for almost all living } \\
\text { organisms. }
\end{array} \\
\hline
\end{array}
\)
In the light of the above statements, choose the most appropriate answer from the options given below :
(d)
“Proteins were conclusively proved to be the genetic material for almost all living organisms.”
❌ This is false.
Proteins were believed (not proved) to be genetic material before Avery’s and Hershey-Chase’s experiments.
But after Hershey-Chase (1952), it became conclusively proven that DNA, not proteins, is the genetic material (in viruses and cells).
So: (R) is false.
Given below are two statements : one is labelled as
Assertion (A) and the other is labelled as Reason (R).
\(
\begin{array}{|l|l|}
\hline \text { Assertion (A): } & \begin{array}{l}
\text { A molecule that can act as a genetic } \\
\text { material should be able to express itself } \\
\text { in the form of ‘Mendelian Characters’. }
\end{array} \\
\hline \text { Reason (R): } & \begin{array}{l}
\text { A molecule that can act as a genetic } \\
\text { material should provide the scope for } \\
\text { slow changes. }
\end{array} \\
\hline
\end{array}
\)
In the light of the above statements, choose the most appropriate answer from the options given below :
(a)
Consider the two statements:-
\(
\begin{array}{|l|l|}
\hline \begin{array}{l}
\text { Statement } \\
\text { I: }
\end{array} & \begin{array}{l}
\text { There is now enough evidence to suggest that } \\
\text { essential life processes (such as metabolism, } \\
\text { translation, splicing, etc.), evolved around } \\
\text { RNA. }
\end{array} \\
\hline \begin{array}{l}
\text { Statement } \\
\text { II: }
\end{array} & \begin{array}{l}
\text { DNA being double-stranded and having } \\
\text { complementary strands further resists changes } \\
\text { by evolving a process of repair. }
\end{array} \\
\hline
\end{array}
\)
(a)
The figure shows the replicating fork during DNA replication. Identify the correct statements.
Statement I: A is an Okazaki fragment and such fragments make up the lagging strand which is replicated away from the replication fork.
Statement II: B is the leading strand that is continuously replicated towards the replication fork.
Who proposed a semi-conservative mode of replication for DNA?
(a)
The daughter DNA that was extracted from the culture one generation after the transfer from \(
{ }^{15} \mathrm{~N} \text { to }{ }^{14} \mathrm{~N}
\) medium had:
(b)
In semi-conservative replication, each daughter DNA molecule gets:
1 old (heavy) strand from 15N{}^{15}\mathrm{N}15N
1 new (light) strand from 14N{}^{14}\mathrm{N}14N
✅ Therefore, both daughter DNA molecules have one heavy and one light strand.
➡️ This makes the DNA have intermediate density, which was observed in cesium chloride (CsCl) gradient centrifugation.
Given below are two statements : One is labelled as Assertion (A) and the other is labelled as Reason (R).
\(
\begin{array}{|l|l|}
\hline \text { Assertion (A): } & \begin{array}{l}
\text { Deoxyribonulceoside triphosphates } \\
\text { provide energy for polymerization } \\
\text { reactions during the replication of DNA. }
\end{array} \\
\hline \text { Reason (R): } & \begin{array}{l}
\text { The two terminal phosphates in a } \\
\text { deoxynucleoside triphosphate are high- } \\
\text { energy phosphates, the same as in the } \\
\text { case of ATP. }
\end{array} \\
\hline
\end{array}
\)
In the light of the above statements, choose the most appropriate answer from the options given below :
(d)
The figure shows the process of transcription in eukaryotes. Identify the incorrect statements regarding the process :
(b)
II:-❌ Why it is incorrect:
2. Wrong mechanism:
✅ Correct version should be:
Polyadenylation adds 200–300 adenylate residues to the 3′ end of pre-mRNA in a template-independent manner.
III:- ❌ Why it is incorrect:
Misleading wording:
Which is wrong — split genes contain both introns (C) and exons (D).
✅ It’s true that C (introns) do not appear in mature RNA,
But saying C “constitute the split gene” is incomplete and misleading.
✅ Correct version should be:
“C are introns, which are part of the split gene and are removed during RNA splicing.”
Consider the following statements :
\(
\begin{array}{|l|l|}
\hline \text { I. } & \begin{array}{l}
\text { Deoxyribonucleoside triphosphates act as substrates } \\
\text { as well as provide energy for polymerization reaction } \\
\text { during DNA replication. }
\end{array} \\
\hline \text { II. } & \begin{array}{l}
\text { The replication of DNA is both semiconservative and } \\
\text { semi-discontinuous }
\end{array} \\
\hline \text { III. } & \begin{array}{l}
\text { In bacterial DNA replication, there are multiple ori } \\
\text { and replication fork moving bi-directionally }
\end{array} \\
\hline
\end{array}
\)
Which of the above statements are true ?
(a)
Statement III :- ❌ False.
Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
\(
\begin{array}{|l|l|}
\hline \text { Assertion (A): } & \begin{array}{l}
\text { For long DNA molecules, the replication } \\
\text { occurs within a small opening of the } \\
\text { DNA helix, referred to as the replication } \\
\text { fork. }
\end{array} \\
\hline \text { Reason (R): } & \begin{array}{l}
\text { The DNA polymerases on their own } \\
\text { cannot initiate the process of replication. }
\end{array} \\
\hline
\end{array}
\)
In the light of the above statements, choose the most appropriate answer from the options given below :
(b)
The DNA dependent polymerases catalyze:
(b)
The codons represented as YYY and ZZZ in the given figure, respectively, can be :
(a)
Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
\(
\begin{array}{|l|l|}
\hline \text { Assertion (A): } & \begin{array}{l}
\text { A piece of DNA if needed to be } \\
\text { propagated during recombinant DNA } \\
\text { procedures requires a vector. }
\end{array} \\
\hline \text { Reason (R): } & \begin{array}{l}
\text { The vectors provide the origin of } \\
\text { replication. }
\end{array} \\
\hline
\end{array}
\)
In the light of the above statements, choose the most appropriate answer from the options given below :
(a)
Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R)
\(
\begin{array}{|l|l|}
\hline \text { Assertion (A): } & \begin{array}{l}
\text { The replication of DNA and cell division } \\
\text { cycle should be highly coordinated. }
\end{array} \\
\hline \text { Reason (R): } & \begin{array}{l}
\text { In eukaryotes, the replication of DNA } \\
\text { takes place at the S-phase of the cell } \\
\text { cycle. }
\end{array} \\
\hline
\end{array}
\)
In the light of the above statements, choose the most appropriate answer from the options given below :
(a)
Let’s rephrase both:
(A): DNA replication and cell division must be synchronized.
(R): DNA replication happens in the S-phase.
➡️ R gives a fact (timing of replication), but it does not directly explain why coordination is needed, or how it’s ensured.
So, while both A and R are correct individually, R doesn’t explain A fully — it states when replication occurs, not why coordination is essential.
What defines a coding and a template strand in the transcription unit ?
(a)
In the given figure Identify A to D :-
\(
\begin{array}{|l|l|l|l|l|}
\hline & \text { A } & \text { B } & \text { C } & \text { D } \\
\hline \text { 1. } & \text { Amino acid binding site } & \text { DHU loop } & \text { Anticodon } & \text { T } \Psi \text { loop } \\
\hline \text { 2. } & \text { Amino acid binding site } & \text { T } \Psi \text { C loop } & \text { Anticodon } & \text { DHU loop } \\
\hline \text { 3. } & \text { Anticodon } & \text { DHU loop } & \text { Amino acid binding site } & \text { T } \Psi \text { C loop } \\
\hline \text { 4. } & \text { Anticodon } & \text { T } \Psi \text { C loop } & \text { Amino acid binding site } & \text { DHU loop } \\
\hline
\end{array}
\)
(c)
Consider the following statements :
\(
\begin{array}{|c|l|}
\hline \text { I. } & \begin{array}{l}
\text { All the reference point while defining a transcription } \\
\text { unit is made with the coding strand. }
\end{array} \\
\hline \text { II. } & \begin{array}{l}
\text { It is the presence of a promoter in a transcription unit } \\
\text { that also defines the template and coding strands. }
\end{array} \\
\hline \text { III. } & \begin{array}{l}
\text { Exons are said to be sequences that do not appear in } \\
\text { mature or processed RNA. }
\end{array} \\
\hline
\end{array}
\)
Which of the above statements are true ?
(a)
III: ❌ False
Exons are expressed regions — they do appear in the mature mRNA after splicing.
It is the introns that are removed during RNA processing.
🔴 Incorrect
Study the diagram of the lac operon given below and select the correct statements :
Statement I: This is the working of a lac operon in the absence of an inducer.
Statement II: Repressor binds to the operator region and allows RNA polymerase to transcribe the operon.
(a)
Statement II :- Repressor binds to the operator region and BLOCK RNA polymerase to transcribe the operon.
Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R)
\(
\begin{array}{|l|l|}
\hline \text { Assertion (A): } & \begin{array}{l}
\text { It is necessary to define the boundaries } \\
\text { that would demarcate the region and the } \\
\text { strand of DNA that would be transcribed. }
\end{array} \\
\hline \text { Reason (R): } & \begin{array}{l}
\text { Reason: Both strands of DNA are not } \\
\text { copied during transcription. }
\end{array} \\
\hline
\end{array}
\)
In the light of the above statements, choose the most appropriate answer from the options given below :
(d)
Because R explains why we choose a strand,
but does not explain why we define the region/boundaries (start and stop of transcription).
Consider the transcription unit given in the following diagram and choose the correct statements :
I. The promoter on the promoter is situated upstream and 5′ to the structural gene.
II. It is the presence of the structural gene that defines the template strand.
III. The coding strand does not code for anything and is displaced during transcription
(b)
Statement II is incorrect because it falsely implies that the gene determines the template strand, whereas in reality, the promoter’s orientation decides which DNA strand will serve as the template.
Detailed Explanantion:-
❌ Why this is incorrect:
- In transcription, the template strand is chosen not by the structural gene itself,
but by the location and orientation of the promoter region.- Promoter sequences are strand-specific and located upstream of the structural gene.
- Once RNA polymerase binds to the promoter, it proceeds in one direction only (3′ → 5′ on the template strand), determining which strand will be used.
Study the diagram given below and choose the correct information that can be deduced :
(a)
Consider the following diagram showing the working of the lac operon in E.coli in the presence of an inducer and choose the correct statement from the ones given below :
(c)
Identity Functions
A Inducer (lactose or allolactose} Binds repressor, inactivates it
B β-galactosidase (from lacZ) Breaks lactose into glucose + galactose
C Permease (from lacY) Helps lactose enter the cell
D Transacetylase (from lacA) Role unclear in lactose metabolism
1. A is the inducer and can be either lactose or cAMP.
→ ❌ Incorrect: Inducer is lactose/allolactose, not cAMP (which regulates via CAP, not the repressor).
2. B is the enzyme that will metabolize glucose, the preferred energy source.
→ ❌ Incorrect: B is β-galactosidase, which metabolizes lactose, not glucose.
3. Some amount of C will be present in the cell even if lactose is absent.
→ ✅ Correct: C is permease, and due to basal expression, it’s present in small amounts always.
4. The absence of D will stop lactose from entering the cell.
→ ❌ Incorrect: D is transacetylase, which has no role in lactose transport; C (permease) does that.
Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R)
\(
\begin{array}{|l|l|}
\hline \text { Assertion (A): } & \begin{array}{l}
\text { Both the strands of DNA are not copied } \\
\text { during transcription. }
\end{array} \\
\hline \text { Reason (R): } & \begin{array}{l}
\text { Both strands of DNA are identical in } \\
\text { their base composition. }
\end{array} \\
\hline
\end{array}
\)
In the light of the above statements, choose the most appropriate answer from the options given below :
(a)
Statement II :❌ False
For example:
❌ So, the Reason is FALSE.
(d)
→ False / Cannot be inferred — The size of chromosomes is not related to VNTR size. VNTR length depends on number of repeat units, not chromosome size.
The diagram does not show any link between chromosome size and VNTR length.
Which of the following is true for both transcription and DNA replication ?
(a)
The DNA profiles below represent four different individuals. Which of the following statements is consistent with the results ?
(b)
Step-by-step matching:
Let’s evaluate Option 3: D is the child of B and C.
Check if each band in D matches either B or C.
Bands in D:
Top band — matches C
2nd band — matches C
3rd band — matches B
4th band — matches C
5th band — matches C
6th band — matches B
7th band — matches C
8th band — matches B
9th band — matches B
✅ All bands in D are present in either B or C → Valid child of B and C
Option 1 (B is child of A and C) ❌ — B has middle bands not matching A or C.
Option 2 (C is child of A and B) ❌ — C has unique bands not found in either A or B.
Option 4 (A is child of B and C) ❌ — Top bands of A don’t match either B or C.
Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R)
\(
\begin{array}{|l|l|}
\hline \text { Assertion (A): } & \begin{array}{l}
\text { In transcription, the strand that does not } \\
\text { code for anything is referred to as the } \\
\text { coding strand. }
\end{array} \\
\hline \text { Reason (R): } & \begin{array}{l}
\text { It is the presence of the promoter in a } \\
\text { transcription unit that defines the } \\
\text { template and coding strands. }
\end{array} \\
\hline
\end{array}
\)
In the light of the above statements, choose the most appropriate answer from the options given below :
(b)
R explains how the strands are selected,
But A makes a statement about the function of the coding strand (that it doesn’t code),
which is not explained or justified by the promoter’s role.
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