Explanation:
The light-independent reactions of photosynthesis (also called the Calvin cycle) do not directly require light but rely on the products (ATP and NADPH) generated during the light-dependent reactions.
The main limiting factors for the light-independent reactions are:
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CO2 concentration: It’s the carbon source for glucose synthesis.
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Temperature: Since the Calvin cycle is enzyme-driven, temperature affects the rate.
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Light: Indirectly influences the Calvin cycle by affecting the production of ATP and NADPH in the light-dependent reactions.
While water and oxygen are crucial for overall plant function, they are not direct limiting factors for the Calvin cycle.
So the best answer is:
1. CO2, temperature, and light