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The inorganic compounds like sulphate, phosphate, etc., are found in
a
The sum total composition of acid-soluble and acid-insoluble fraction represents the entire composition of
c
Biomolecules are
c
Which of the following options correctly identifies the structural formulae shown in figure?
(c) Glucose and ribose both are carbohydrates. Ribose sugar constitutes RNA nucleotides. Glucose is a hexose sugar while ribose is a pentose sugar.
Identify the amino acids given below and select the correct option.
b
The 20 different amino acids have different
a
Which of the following is the correct match?
\(
\begin{array}{|l|l|l|}
\hline {\begin{array}{c}
\text { Acidic } \\
\text { amino acid }
\end{array}} & {\begin{array}{c}
\text { Basic } \\
\text { amino acid }
\end{array}} & {\begin{array}{c}
\text { Neutral } \\
\text { amino acid }
\end{array}} \\
\hline \text {a. Glutamic acid } & \text { Lysine } & \text { Valine } \\
\hline \text {b. Lysine } & \text { Valine } & \text { Glutamic acid } \\
\hline \text {c. Glutamic acid } & \text { Valine } & \text { Lysine } \\
\hline \text {d. Lysine } & \text { Glutamic acid } & \text { Valine } \\
\hline
\end{array}
\)
a
Acidic amino acids have two \(-\mathrm{COOH}\) groups and one \(-\mathrm{NH}_2\) group per molecule. Select the pair that consists of acidic amino acids.
(a) Lysine and arginine are basic amino acids whereas glycine and alanine are neutral amino acids.
An example of aromatic amino acid is
(d) Aromatic amino acids possess cyclic structure in the side chain, e.g., phenylalanine, tryptophan (actually heterocyclic) or tyrosine (having \(\mathrm{OH}\) group).
Saturated fatty acids possess _____ bonds between carbon atoms and are ____ at room temperature.
(a) Fatty acids are of two main types- saturated and unsaturated. Saturated fatty acids are without double bonds in their carbon chains. They have higher melting points and are solids at room temperature. They have a general formula of \(\mathrm{C}_n \mathrm{H}_{2 n} \mathrm{O}_2\). Animal fats are mostly saturated fats.
Unsaturated fatty acids contain one or more double bonds in their carbon chains. They have lower melting points and are liquids at room temperature. Their general formula is \(\mathrm{C}_n \mathrm{H}_{2 n-2 \times} \mathrm{O}_2\). Plant fats are generally unsaturated fats.
Which of the following is a saturated fatty acid?
d
Triglycerides are fatty acid esters of glycerol, which are formed by the esterification of ____ molecules of fatty acids with ____ molecule(s) of glycerol.
(b) The three hydroxyl groups of a glycerol molecule join with the carboxyl groups of three fatty acid molecules to form a fat or triglyceride molecule. The chemical linkage between the glycerol and fatty acids is called the ester bond. It results from the elimination of three molecules of water (condensation or dehydration).
Which of the following is a triglyceride?
(c) Hard fats (e.g., butter, ghee) and oils (e.g., sunflower oil, mustard oil, groundnut oil, etc.) are examples of triglycerides.
Given molecular formula belongs to which of the following groups of biomolecules?
d
Given structural formula is correctly identified along with its related function by which of the following options?
(a) Cholesterol is the most abundant steroid in the animal tissues. It is present in food especially that rich in animal fats. It is an essential component of the animal plasma membrane as it regulates the fluidity of membrane.
Given structural formula is correctly identified along with its related function by which of the following options?
b
Lecithin is a
(c) Phospholipids are triglyceride lipids, in which one fatty acid is replaced by phosphoric acid residue often linked to additional nitrogenous groups, e.g., choline in lecithin.
An unknown liquid collected from a sample of peas is added to a beaker of water and is vigorously shaken. After few minutes, water and the unknown liquid made two separate layers. To which class of biomolecules, does the unknown liquid most likely belongs?
(c) Since lipids are mainly non-polar, they are immiscible with water, which is polar.
The component present in both nucleotides and nucleosides is
(d) Nucleoside is a compound formed by the union of a nitrogen base with a pentose sugar. It is a component of nucleotide. Each nucleotide is composed of three units; a nitrogen base, a pentose sugar and a phosphate group.
Cytidine is a
(b) Cytosine (C) is a pyrimidine i.e., a nitrogenous base. A combination of a nitrogenous base (purine or pyrimidine) with a pentose sugar is known as nucleoside. Thus, the combination of cytosine with ribose sugar results in the formation of a nucleoside called as cytidine. Similarly, the combination of cytosine with deoxyribose sugar is called as deoxycytidine.
Refer to the given reactions.
(i) Adenine \(+X \rightarrow\) Adenosine
(ii) Adenosine \(+Y \rightarrow\) Adenylic acid
What does \(X\) and \(Y\) represent here?
(b)
Identify the given structural formulae and select the correct option.
(a) Nitrogen bases are heterocyclic compounds. They are of two types, substituted purines and substituted pyrimidines. Purines are larger-sized nitrogen-containing biomolecules. They have 9 -9-membered double rings. A purine has imidazole ring joined to pyrimidine ring at 4 and 5 positions. It has nitrogens at 1,3, 7 and 9 positions. There are two types of purines – adenine (A) and guanine (G). Pyrimidines are 6 -membered rings. A pyrimidine ring has nitrogen at 1 and 3 positions. Pyrimidine bases are of three types – cytosine (C), thymine (T) and uracil (U).
The three structural formulae A, B and C are given here. Identify them and select the correct option.
b
Adenosine, guanosine, thymidine, uridine, cytidine are all ____ but adenylic acid, guanylic acid, uridylic acid, cytidylic acid are ____.
b
Match column I with column II and select the correct option from the given codes.
\(
\begin{array}{|l|l|c|l|}
\hline & \begin{array}{l}
\text { Column I } \\
\text { (Category) }
\end{array} & & \begin{array}{l}
\text { Column II } \\
\text { (Secondary metabolites) }
\end{array} \\
\hline \text { A. } & \text { Pigments } & \text { (i) } & \text { Concanavalin A } \\
\hline \text { B. } & \text { Terpenoides } & \text { (ii) } & \text { Monoterpenes, diterpenes } \\
\hline \text { C. } & \text { Alkaloids } & \text { (iii) } & \text { Morphine, codeine } \\
\hline \text { D. } & \text { Lectins } & \text { (iv) } & \text { Carotenoids, anthocyanins } \\
\hline
\end{array}
\)
a
Which of the following secondary metabolites are used as drugs?
(b) Vinblastin is used as an anticancer drug whereas curcumin is a component of turmeric and also used as a drug.
Which of the following are alkaloids?
(d) Codeine and morphine are alkaloids whereas cellulose is polymeric substance.
Select the incorrect match from the following.
(c) Anthocyanins are pigments.
Study the given statements and select the correct option.
(i) Carbohydrates, proteins, nucleic acids and lipids are primary metabolites.
(ii) Alkaloids, flavonoids, rubber, etc., are secondary metabolites.
(iii) Linoleic, linolenic and palmitic acids are the three essential fatty acids.
(a) Linoleic, linolenic and arachidonic acids are essential fatty acid.
Which of the following is polymeric compound?
b
The correct order of chemical composition of living tissues/cells in term of percentage of the total cellular mass is
(b) The average composition of a living cell is water 70 \(90 \%\); proteins \(10-15 \%\); nucleic acids \(5-7 \%\); carbohydrates \(3 \%\); lipids \(2 \%\); ions \(1 \%\).
Match the column I with column II and choose the correct combination from the given options.
\(
\begin{array}{|c|l|c|c|}
\hline & \begin{array}{l}
\text { Column I } \\
\text { (Component) }
\end{array} & & \begin{array}{l}
\text { Column II } \\
\text { (% of the total } \\
\text { cellular mass) }
\end{array} \\
\hline \text { A. } & \text { lons } & \text { (i) } & 1 \\
\hline \text { B. } & \text { Lipids } & \text { (ii) } & 2 \\
\hline \text { C. } & \text { Carbohydrates } & \text { (iii) } & 3 \\
\hline \text { D. } & \text { Nucleic acids } & \text { (iv) } & 5-7 \\
\hline \text { E. } & \text { Proteins } & \text { (v) } & 10-15 \\
\hline
\end{array}
\)
(a) The average composition of a living cell is water 70 \(90 \%\); proteins \(10-15 \%\); nucleic acids \(5-7 \%\); carbohydrates \(3 \%\); lipids \(2 \%\); ions \(1 \%\).
Which of the following organic compounds is/are acid insoluble fraction?
(i) Proteins
(ii) Nucleic acids
(iii) Polysaccharides
(iv) Lipids
d
Which of the following organic compounds is acid insoluble fraction but molecular weights do not exceed 800 Da?
d
Select the incorrect match regarding average composition of cells.
\(
\begin{array}{|l|c|}
\hline {\text { Component }} & \begin{array}{c}
\text { % of the total Cellular } \\
\text { mass }
\end{array} \\
\hline \text { Water } & 70-90 \\
\hline \text { Lipids } & 2 \\
\hline \text { Nucleic acids } & 1 \\
\hline \text { Proteins } & 10-15 \\
\hline
\end{array}
\)
(c) Nucleic acids percentage (%) of the total cellular mass is \(5.7 \%\).
Read the given statements and select the option that correctly sorts these with respect to \(A\) and \(B\) in the given flow chart.
(i) Molecular weight ranging from 18 to 800 daltons (Da) approximately
(ii) Proteins, nucleic acids, polysaccharides and lipids
(iii) Contain chemicals that have molecular weight more than 800 Da
(iv) Has monomers
(v) Generally has polymers
c
Take a living tissue, grind it in trichloroacetic acid using pestle and mortar, and then strain it, you would obtain two fractions: acid-soluble and acid-insoluble fraction. Acid-insoluble fraction does not contain
(d) The acid-soluble pool represents roughly the cytoplasmic composition whereas the macromolecules from cytoplasm and organelles become the acid-insoluble fraction. Together they represent the entire chemical composition of living tissues of organisms or cellular pool. All the compounds which are found in the acid-soluble pool, have their molecular weights ranging from approx. 18 to around 800 daltons (Da).
The acid-insoluble fraction, has only four types of organic compounds i.e., proteins, nucleic acids, polysaccharides and lipid.
Match column I with column II and select the correct option from the given codes.
\(
\begin{array}{|c|l|c|l|}
\hline & \text { Column I } & & \text { Column II } \\
\hline \text { A. } & \text { GLUT-4 } & \text { (i) } & \text { Intercellular ground substance } \\
\hline \text { B. } & \text { Antibody } & \text { (ii) } & \text { Enzyme } \\
\hline \text { C. } & \text { Collagen } & \text { (iii) } & \text { Hormone } \\
\hline \text { D. } & \text { Trypsin } & \text { (iv) } & \text { Fights infectious agents } \\
\hline \text { E. } & \text { Insulin } & \text { (v) } & \begin{array}{l}
\text { Enables glucose transport in } \\
\text { cells }
\end{array} \\
\hline
\end{array}
\)
b
_____ is the most abundant protein in animal world and ____ is the most abundant protein in the whole biosphere.
(a) Keratin is a fibrous protein which forms external protective structures of animals like nails, hoofs, scales, hair, feathers, horny layer of skin, etc.
What will be the molecular formula of a polypeptide consisting of 10 glycine molecules when the formula of glycine is \(\mathrm{C}_2 \mathrm{H}_5 \mathrm{O}_2 \mathrm{~N}\)?
(b) A polypeptide is formed by the condensation of glycine monomers, i.e., by the elimination of one water molecule during the formation of each peptide bond. When 10 glycine \(\left(\mathrm{C}_2 \mathrm{H}_5 \mathrm{O}_2 \mathrm{~N}\right)\), molecules participate, \(9 \mathrm{H}_2 \mathrm{O}\) molecules are eliminated. Thus resulting polypeptide will possess the molecular formula \(\mathrm{C}_{20} \mathrm{H}_{32} \mathrm{O}_{11} \mathrm{~N}_{10}\).
Which of the following is correct group of protein?
(a) Lecithin is a phospholipid.
Which of the following is not a homopolymer?
a
Essential amino acids include
(d) Essential amino acids are those which cannot be synthesised by organisms in the body and are obtained from plants, e.g., valine, leucine, isoleucine, phenylalanine, threonine, lysine, tryptophan, methionine.
Non-essential amino acids can be synthesised by the organism and may not be the requisite components of diet, e.g., serine, cysteine, proline, glycine, alanine, asparagine, glutamine and tyrosine.
Which of the following statements about amino acids is incorrect?
(d) Acidic amino acids have an extra carboxylic group (mono-amino dicarboxylic), e.g., glutamate (glutamic acid or Glu), asparate (aspartic acid or Asp).
Basic amino acids have an additional amino group without forming amides (diamino monocarboxylic), e.g., arginine (Arg), lysine (Lys).
The polysaccharides made up of glucose monomers are
c
Study the given statements and select the correct answer.
(i) Cellulose is a homopolymer of glucose.
(ii) Inulin is a homopolymer of fructose.
(iii) Starch gives blue colour with iodine solution.
(iv) Cellulose gives no colour with iodine solution.
(d) Starch can hold \(I_2\) (iodine) molecules in the helical portion. The starch- \(\mathrm{I}_2\) is blue in colour. Cellulose does not contain complex helices and hence cannot hold \(\)\mathrm{I}_2\(\) and hence remain colourless.
Read the given statements and select the correct option.
(i) Right end of a polysaccharide chain is called reducing end while left end is called non-reducing end.
(ii) Starch can hold iodine molecules in its helical secondary structure but cellulose being non-helical, cannot hold iodine.
(iii) Starch and glycogen are branched molecules.
(iv) Starch and glycogen are the reserve food materials of plants and animals, respectively.
d
Which of the following is a heteropolymer?
(c) Homopolysaccharides or homoglycans are those complex carbohydrates which are formed by polymerisation of only one type of monosaccharide monomers. For example, starch, glycogen and cellulose are composed of a single type of monosaccharide called glucose.
Heteropolysaccharides or heteroglycans are those complex carbohydrates which are produced by condensation of two or more types of monosaccharide derivatives, e.g., agar, peptidoglycan, glycosaminoglycans, etc.
Which of the following statements is not correct regarding chitin?
(a) Chitin is the second most abundant polysaccharide (after cellulose). Chitin is not a storage polysaccharide rather it is a structural homopolysaccharide, which forms the structural component of fungal walls and exoskeleton of arthropods, It is an unbranched polysaccharide formed of \(\mathrm{N}\)-acetylglucosamine (NAG) monomers joined together by \(\beta-1,4\) linkages.
Read the given statements and select the correct ones.
(i) Fructose is the sweetest sugar.
(ii) Glycine is the simplest amino acid.
(iii) Lactose is a disaccharide composed of one molecule each of glucose and galactose.
(iv) Cellulose is an unbranched chain of glucose molecules linked by \(\beta-1\), 4-glycosidic bond.
d
Purines have nitrogen atoms at ____ positions.
(a) Nitrogen bases are heterocyclic compounds. They are of two types, substituted purines and substituted pyrimidines. Purines are larger-sized nitrogen-containing biomolecules. They have 9 -9-membered double rings. A purine has imidazole ring joined to pyrimidine ring at 4 and 5 positions. It has nitrogens at 1,3, 7 and 9 positions. There are two types of purines – adenine (A) and guanine (G). Pyrimidines are 6 -membered rings. A pyrimidine ring has nitrogen at 1 and 3 positions. Pyrimidine bases are of three types – cytosine (C), thymine (T) and uracil (U).
Pyrimidines have nitrogen atoms at ____ positions.
(c) Nitrogen bases are heterocyclic compounds. They are of two types, substituted purines and substituted pyrimidines. Purines are larger-sized nitrogen-containing biomolecules. They have 9 -9-membered double rings. A purine has imidazole ring joined to pyrimidine ring at 4 and 5 positions. It has nitrogens at 1,3, 7 and 9 positions. There are two types of purines – adenine (A) and guanine (G). Pyrimidines are 6 -membered rings. A pyrimidine ring has nitrogen at 1 and 3 positions. Pyrimidine bases are of three types – cytosine (C), thymine (T) and uracil (U).
Which of the following is an incorrect match?
(c) Inulin is a fructan storage polysaccharide of roots and tubers of Dahlia and related plants. Inulin is not metabolised in human body and is readily filtered through the kidney. It is, therefore, used in testing of kidney function, especially glomerular filtration.
The three chemically distinct components of a nucleotide are
a
Which of the following is correct group of pyrimidine?
c
\(
\text { Identify } X \text { and } Y \text { in the given sequence. }
\)
(a) While representing the primary structure of a protein, the N-terminal amino acid is always written on the first (left) end of the polypeptide chain and C-terminal amino acid at the right end of the chain.
At some points a protein chain may be folded back on itself like a hollow woollen ball. This is called ___ structure and folds or coils are held together in place by ___.
c
An \(\)\alpha\(\)-helix is the example of which type of protein structure?
c
A \(\beta\)-pleated sheet organisation in a polypeptide chain is an example of
c
Refer to the given figure.
Formation of structures A and B could be due to
\(
\begin{array}{|c|c|}
\hline \text { A } & \text { B } \\
\hline \begin{array}{l}
\text { a. Formation of peptide } \\
\text { bonds }
\end{array} & \begin{array}{l}
\text { Linking together of two } \\
\text { or more polypeptides }
\end{array} \\
\hline \begin{array}{l}
\text { b. Formation of hydrogen } \\
\text { bonds }
\end{array} & \begin{array}{l}
\text { Linking together of two } \\
\text { or more polypeptides }
\end{array} \\
\hline \begin{array}{l}
\text { c. Formation of hydrogen } \\
\text { bonds }
\end{array} & \begin{array}{l}
\text { Formation of hydrogen } \\
\text { bonds }
\end{array} \\
\hline \begin{array}{l}
\text { d. Formation of peptide } \\
\text { bonds }
\end{array} & \begin{array}{l}
\text { Formation of peptide } \\
\text { bonds }
\end{array} \\
\hline
\end{array}
\)
(b) The folding of a linear polypeptide chain into a specific coiled structure is referred to as the secondary structure of the protein. Such coiling or folding is produced by hydrogen bonds. The importance of such folding lies in the fact that amino acid residues that may lie far apart in their primary sequence are brought together in close proximity by secondary folding. There are three types of secondary structures- \(\alpha\)-helix, \(\beta\)-pleated sheets and collagen helix.
In \(\alpha\)-helix, hydrogen bonds between oxygen of carboxylic group of one amino acid residue and NH group of next amino acid residue. In \(\beta\)-pleated secondary structure, two or more polypeptide chains get interconnected by hydrogen bonds. Adjacent strands of polypeptides may run in the same direction (parallel \(\beta\)-sheet, e.g., \(\beta\)-keratin) or in opposite directions (antiparallel \(\beta\)-sheet, e.g., fibroin of silk).
Recognise the figure and find out the correct matching.
c
Adult human haemolgobin consists of
c
Read the given statements and select the correct option.
Statement 1: Haemoglobin is an example of quaternary structure of proteins.
Statement 2: Haemoglobin molecule is composed of four polypeptide chains-two \(\alpha\)-chains and two \(\beta\)-chains.
(a) Proteins are said to have quaternary structure if they consist of 2 or more polypeptide chains united by forces other than covalent bonds (i.e., not peptide or disulphide bonds). The forces that stabilise these aggregates are hydrogen bonds and electrostatic bonds formed between amino acids on the polypeptide chains. Haemoglobin, the oxygen-transporting protein of blood, is an example of quaternary structure. This protein consists of four peptide chains of two types- \(\alpha\) and \(\beta\) chains that occur in pairs.
Keratin present in hair shows secondary structure known as
(c) \(\alpha\)-helical coiled secondary structure is found in several protein, e.g., keratin, myosin, epidermin, fibrin, etc.
Select the option that correctly identifies the chemical bonds present in the given biomolecules.
Polysaccharides – A, Proteins – B, Fats – C, Water – D
(b) In polysaccharides, monosaccharides are linked together by glycosidic bond \((\mathrm{C}-\mathrm{O}-\mathrm{C})\). It is formed by dehydration between two carbon atoms of two adjacent monosaccharides. Water has hydrogen bonding. Amino acids in a protein are linked through peptide bond whereas ester bond is present in fats
What does A represent in the given diagram of a nucleotide?
c
Which bonds are indicated by X and Y in the given diagram?
b
Primary structure of proteins is due to the presence of
a
Which of the two groups of the given formula is involved in peptide bond formation between different amino acids?
(b) Proteins are polymers of different amino acids, which are linked by peptide (CONH) bond formed by dehydration (polymerisation) between \(\mathrm{COOH}\) group of one amino acid and \(\mathrm{NH}_2\) group of next amino acid with the removal of \(\mathrm{H}_2 \mathrm{O}\).
B-DNA which is right-handed double helix contains ____ base pairs per turn of the helix and each turn is ____ long.
(b) In B-DNA, one turn of the helix has about 10 nucleotides on each strand of DNA. A turn occupies a distance of about 3.4 nm (34 Å or \(\left.3.4 \times 10^{-9} \mathrm{~m}\right)\) so that adjacent nucleotides or their bases are separated by a space of about \(0.34 \mathrm{~nm}\left(0.34 \times 10^{-9}\right.\) \(\mathrm{m}\) or 3.4 Å).
Read the given statement and select the option that correctly identifies X and Y.
In a glycogen molecule, successive glucose units are joined together by X and branches are linked together by Y.
b
Read the following statements and select the correct option.
Statement 1: All biomolecules have a turnover.
Statement 2: One type of biomolecule changes into some other type of biomolecule.
(a) All biomolecules have a turnover. This means that they are constantly being changed into some other biomolecules and also made from some other biomolecules.
The proteins which hasten the rate of a given metabolic conversion are called
(c) The catalysts which hasten the rate of a metabolic conversion are proteins. These proteins with catalytic power are named enzymes.
Which of the following is an example of metabolic transformation?
d
The catalysts which hasten the rate of a metabolic conversion are
(c) The catalysts which hasten the rate of a metabolic conversion are proteins. These proteins with catalytic power are named enzymes.
Select the correct statement.
(c) Metabolic pathways are either linear or circular. \(\mathrm{CO}_2\) dissolving in water is catalysed reaction in living system as there is no uncatalysed metabolic conversion in living systems. Metabolic reactions are chemical reactions.
The most important form of energy currency in living systems is the bond energy in a chemical called
a
Select the correct statement regarding metabolic pathway in which acetic acid is converted into cholesterol.
c
Glucose becomes lactic acid in our skeletal muscles is an example of
(b) Glucose becomes lactic acid in our skeletal muscles is metabolic pathway in which energy is liberated.
Anabolic pathway in metabolism are (i) pathway and (ii) energy. Select the option that correctly fills blanks (i) and (ii).
c
Which of the following processes requires energy input?
d
The blood concentration of glucose in a normal healthy individual is
b
The living state is a ____ perform work. state to be able to
(a) The living state is a non-equilibrium steady state to be able to perform work.
The blood concentration of hormone in a normal healthy individual would be measured in
c
Living process is a constant effort to prevent falling into (i). This is achieved by energy (ii). Select the option that correctly fill blanks (i) and (ii).
a
Select the incorrect statement.
(b) The system at equilibrium cannot perform work.
Enzymes catalyse the biochemical reactions by ____ the activation energy.
a
Which of the following statements about enzymes are correct?
(i) Enzymes do not alter the overall change in free energy for a reaction.
(ii) Enzymes are proteins whose three dimensional shape is key to their functions.
(iii) Enzymes speed up reactions by lowering activation energy.
(iv) Enzymes are highly specific for reactions.
(v) The energy input needed to start a chemical reaction is called activation energy.
d
Read the given statements and select the correct option.
Statement 1: Ribozymes are RNA molecules which catalyse the synthesis of certain specific RNAs and removal of introns from mRNA.
Statement 2: Ribozymes are proteinaceous enzymes.
(b) Ribozymes are a group of ribonucleic acids that function as biological catalysts and are regarded as non-protein enzymes.
\(
\text { What is denoted by } X \text { and } Y \text { in the given graph? }
\)
\(
\begin{array}{|l|l|}
\hline {\mathbf{X}} & {\mathbf{Y}} \\
\hline \begin{array}{l}
\text {a. Activation energy } \\
\text { without enzyme }
\end{array} & \begin{array}{l}
\text { Activation energy with } \\
\text { enzyme }
\end{array} \\
\hline \begin{array}{l}
\text {b. Activation energy } \\
\text { with enzyme }
\end{array} & \begin{array}{l}
\text { Activation energy } \\
\text { without enzyme }
\end{array} \\
\hline \begin{array}{l}
\text {c. Substrate } \\
\text { concentration with } \\
\text { enzyme }
\end{array} & \begin{array}{l}
\text { Substrate concentration } \\
\text { without enzyme }
\end{array} \\
\hline \begin{array}{l}
\text {d. Substrate } \\
\text { concentration } \\
\text { without enzyme }
\end{array} & \begin{array}{l}
\text { Substrate concentration } \\
\text { with enzyme }
\end{array} \\
\hline
\end{array}
\)
(b) Most reactions do not start automatically because the reactions have an energy barrier which may be due to hydrogen bonding, absence of precise collisions due to small reactive site, mutual repulsion, etc. External supply of energy needed to overcome this barrier is known as activation energy. The enzyme lowers the activation energy of the reaction and allows a large number of molecules to react at a time.
Which of the following statements is incorrect regarding enzymatic activity?
(c) Every enzyme possesses an optimum pH value, where it is most effective. Most enzymes show maximum activity in a \(\mathrm{pH}\) range of about 6.0 to 7.5 , i.e, near neutral pH. Some digestive enzymes have their optimum pH in the acidic or alkaline range. E.g., pepsin of gastric juice has its optimum at \(\mathrm{pH} ~2\) (acidic) whereas trypsin of pancreatic juice shows maximum activity at \(\mathrm{pH} ~8.8\) (alkaline). A rise and fall in \(\)\mathrm{pH}\(\) reduces enzyme activity by changing the degree of ionisation of its side chain.
Read the given statements and select the correct option.
Statement 1: Low temperature destroys enzymes by causing their denaturation.
Statement 2: High temperature preserves the enzymes in their inactive stage.
(d) Low temperature preserves the enzymes in their inactive state. Therefore, it is used in preservation of foods inside cold storages. Low temperature present inside cold storages prevents spoilage of food.
High temperature destroys enzymes by causing their denaturation. This occurs at \(50^{\circ} \mathrm{C}\) or so. In between the minimum and maximum temperatures, the reaction velocity doubles for every \(10^{\circ} \mathrm{C}\) rise in temperature (general rule of thumb).
The given graph represents effects of change in various factors on enzymatic activity. Select the option that correctly identifies X-axis and Y-axis.
d
Which one of the given graphs shows the effect of \(\mathrm{pH}\) on the velocity of a typical enzymatic reaction (V)?
c
Which one of the given graphs shows the effect of temperature on the velocity of a typical enzymatic reaction?
c
Michaelis Menten Constant \(\left(\mathrm{K}_m\right)\) is equal to
(c) : \(\mathrm{K}_m\) or the Michaelis-Menten constant is defined as the substrate concentration (expressed in moles/l) at which halfmaximum velocity in an enzyme catalysed reaction is achieved. It indicates that half of the enzyme molecules (i.e. \(50 \%\) ) are bound with the substrate molecules when the substrate concentration equals the \(K_m\) value. \(K_m\) value is a characteristic feature of a given enzyme. A low \(K_m\) value indicates a strong affinity between enzyme and substrate, whereas a high \(K_m\) value reflects a weak affinity between them.
Enzymes are most functional within the temperature range of
b
The inhibitor which closely resembles the substrate in its molecular structure and inhibits the enzyme activity by binding to the active site of the enzyme is called
(c) In competitive inhibition, the inhibitor (I) closely resembles the real substrate (S), and is regarded as a substrate analogue. The inhibitor competes with substrate and binds at the active site of the enzyme but does not undergo any catalysis. As long as the competitive inhibitor holds the active site, the enzyme is not available for the substrate to bind. A competitive inhibitor diminishes the rate of catalysis by reducing the proportion of enzyme molecules bound to a substrate.
Inhibition of succinate dehydrogenase by malonate is an example of
b
Feedback inhibition of an enzyme is influenced by
(b) In non-competitive inhibition, the inhibitor binds at a site other than the active site on the enzyme surface. This binding impairs the enzyme function. The inhibitor has no structural resemblance with the substrate. It does not interfere with the enzyme-substrate binding but the catalysis is prevented, possibly due to a distortion in the enzyme conformation.
Feedback inhibition of an enzyme is influenced by
(c) In feedback inhibition, product of a reaction inhibits the enzyme catalysing that reaction. It is a type of control mechanism at the enzyme level. If the product is produced in sufficient amount, it inhibits the enzyme to stop the further production.
Refer to the given reaction.
\(\)
\mathrm{C}_{12} \mathrm{H}_{22} \mathrm{O}_{11}+\mathrm{H}_2 \mathrm{O} \stackrel{\text { Enzyme } \mathrm{A}}{\longrightarrow} 2 \mathrm{C}_6 \mathrm{H}_{12} \mathrm{O}_6
\(\)
Enzyme A used in the reaction, belongs to which class of enzymes?
(c) Hydrolases break up large molecules into smaller ones with the help of hydrogen and hydroxyl groups of water molecules. The phenomenon is called hydrolysis. Digestive enzymes belonging to this group are amylase (hydrolysis of starch), sucrase, lactase, etc.
Enzymes that catalyse removal of gróups from substrates by mechanisms other than hydrolysis, and addition of groups to double bonds, are called
b
Dihydroxyacetone-3-phosphate and glyceraldehyde-3 phosphate are interconvertible. The enzyme responsible for this interconversion belongs to the cateogry of
a
Which of the following is an example of isozyme?
(d) The multiple molecular forms of an enzyme occurring in the same organism and having a similar substrate activity are called isoenzymes or isozymes. They have similar properties but different molecular weights and locations. Over 100 enzymes are known to have isoenzymes. \(\alpha\)-amylase of wheat endosperm has 16 isozymes, lactate dehydrogenase has 5 isozymes. Glucokinase is an isozyme of hexokinase.
Holoenzyme is the complete enzyme consisting of an apoenzyme and a co-factor. Select the option that correctly identifies the nature of apoenzyme and co-factor.
\(
\begin{array}{|l|l|}
\hline{\text { Apoenzyme }} & {\text { Co-factor }} \\
\hline \text {a. Protein } & \text { Non-protein } \\
\hline \text {b. Non-protein } & \text { Protein } \\
\hline \text {c. Protein } & \text { Protein } \\
\hline \text {d. Non-protein } & \text { Non-protein } \\
\hline
\end{array}
\)
(a) Enzymes may be broadly classified into two types depending on their chemical composition – simple enzymes and conjugated enzymes. Simple enzymes are wholly made up of proteins and any additional substance or group is absent, e.g., pepsin, trypsin, etc. Conjugated enzymes (or holoenzymes) are formed of two parts – a protein part called apoenzyme and a non-protein part named co-factor. The complete conjugated enzyme consisting of an apoenzyme and a co-factor is called holoenzyme. Holoenzyme is the functional unit of enzyme.
Holoenzyme \(\longrightarrow\) Apoenzyme + Co-factor
(Active enzyme) (Protein part) (Non-protein part)
Co-factor may be inorganic or organic in nature. Catalytic activity is lost when co-factor is removed from the enzyme which indicates that it plays a crucial role in catalytic activity of enzymes.
Read the given paragraph with few blanks. Prosthetic groups are (i) compounds distinguished from other co-factors in being (ii) bound to the apoenzyme. For example, in peroxidase and (iii) which catalyse the breakdown of hydrogen peroxide to water and (iv), (v) is the prosthetic group. Select the option that correctly fills blanks in the above paragraph.
\(
\begin{array}{|l|l|l|l|l|l|}
\hline & \text { (i) } & \text { (ii) } & \text { (iii) } & \text { (iv) } & \text { (v) } \\
\hline \text { (a) } & \text { organic } & \text { tightly } & \text { catalase } & \text { oxygen } & \text { haem } \\
\hline \text { (b) } & \text { inorganic } & \text { loosely } & \text { catalase } & \text { hydrogen } & \text { haem } \\
\hline \text { (c) } & \text { inorganic } & \text { tightly } & \text { isomerase } & \text { hydrogen } & \text { haem } \\
\hline \text { (d) } & \text { organic } & \text { loosely } & \text { isomerase } & \text { oxygen } & \text { haem } \\
\hline
\end{array}
\)
a
Co-enzyme nicotinamide adenine dinucleotide (NAD) contains vitamin
(c) Most of the co-enzymes are made up of water-soluble vitamins ( B and C ), e.g., thiamine, nicotinamide, etc. Co-enzyme NAD and NADP contain niacin vitamins.
Zinc is a co-factor for proteolytic enzyme ___.
a
Read the given statements and select the correct option.
Statement 1: Co-factors play a crucial role in the catalytic activity of the enzyme.
Statement 2: Catalytic activity is lost when co-factor is removed from the enzyme.
a
Biochemical reagents are widely used for detection of biomolecules. A reagent that specifically detects a carbonyl group \((C=O)\) in a biomolecule will yield a positive test with
b
Which of the following graphs correctly indicates the reaction in presence (indicated by + ) and absence (indicated as -) of an enzyme?
d
The standard free energy change and standard activation energy for four biochemical reactions are listed in the table below.
\(
\begin{array}{|c|c|c|}
\hline \text { Reaction } & \begin{array}{c}
\text { Standard free } \\
\text { energy change } \\
\text { (kcal/mol) }
\end{array} & \begin{array}{c}
\text { Standard } \\
\text { activation energy } \\
\text { (kcal/mol) }
\end{array} \\
\hline P & -40 & 18 \\
\hline Q & -71 & 18 \\
\hline R & -40 & 11 \\
\hline S & -71 & 11 \\
\hline
\end{array}
\)
A few interpretations are given below. Among these, the most appropriate interpretation is
(d) Standard free energy represents the energy available to do work at standard temperature. Activation energy represents the energy required to start a reaction. The enzyme works by lowering down the activation energy.
Refer to the given graph showing the relationship between temperature and enzyme action.
Select the correct statement regarding ‘ A ‘ and ‘ B ‘.
(i) ‘ A ‘ shows rate at which reaction decreases due to denaturation of enzyme molecules.
(ii) ‘ B ‘ shows rate at which reaction increases due to decreased kinetic energy of substrate.
(iii) As temperature rises, more and more enzyme molecules are denatured and ‘ A ‘ appears to fall.
(iv) ‘ B ‘ shows rate at which reaction increases due to increased kinetic energy of substrate and enzyme molecules.
(a) In this study, enzyme and substrate are treated with increasing temperature before they are mixed, and the rate of reaction is determined. Enzymes are mostly proteinaceous molecules. Proteins are denatured by heat, and the rate of denaturation increases and becomes significant at higher temperatures. When enzyme is mixed with substrate, with increase in temperature the rate of reaction increases as there is increased kinetic energy of both substrate and enzyme molecules leading to more rapid movement and their collisions. However, with increase in temperature denaturation of enzyme occurs which is an irreversible destruction; eventually destroying the active sites. As a result there is an apparent optimum temperature for the action of an enzyme due to dual effects, firstly, on the reaction and secondly, the stability of the enzyme protein.
Refer to the given figure of a polysaccharide and select the incorrect option regarding it.
(c) Given figure is of amylopectin (a component of starch). It gives red-violet colour with iodine solution.
How many of the following fatty acids are essential fatty acids?
Palmitic acid, stearic acid, linoleic acid, arachidonic acid, linolenic acid
(d) Essential fatty acids must be present in animal (human) diet. Animals lack the mechanism to synthesise essential fatty acids which include – linoleic acid, linolenic acid and arachidonic acid. These three fatty acids occur in most edible oils like sunflower, groundnut, coconut, cotton seed, etc. Their deficiency produces phrynoderma or follicular hyperkeratosis.
The given table shows differences between apoenzyme and coenzyme. Select the incorrect pair of differences.
\(
\begin{array}{|c|c|c|}
\hline & \text { Apoenzyme } & \text { Coenzyme } \\
\hline \text { (i) } & \begin{array}{l}
\text { It is protein part } \\
\text { of the holoenzyme } \\
\text { or conjugate } \\
\text { enzyme. }
\end{array} & \begin{array}{l}
\text { It is the nonprotein organic } \\
\text { group which gets attached to the } \\
\text { apoenzyme to from holoenzyme } \\
\text { or conjugate enzyme. }
\end{array} \\
\hline \text { (ii) } & \text { It is small in size. } & \text { It is large in size. } \\
\hline \text { (iii) } & \begin{array}{l}
\text { It takespartin only } \\
\text { catalyticactivity of } \\
\text { the enzyme. }
\end{array} & \begin{array}{l}
\text { It helps in removing a product } \\
\text { of chemical reaction besides } \\
\text { bringing contact between the } \\
\text { substrate and the enzyme. }
\end{array} \\
\hline \text { (iv) } & \text { It is heat stable. } & \text { It is thermolabile. } \\
\hline
\end{array}
\)
(d) Apoenzyme is large in size while co-enzyme is small in size. Apoenzyme is thermolabile while coenzyme is heat stable.
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