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\(
\begin{aligned}
&\begin{array}{|c|c|c|c|}
\hline & \text { List I } & & \text { List II } \\
\hline \text { A. } & \text { Clostridium butylicum } & \text { ।. } & \text { Ethanol } \\
\hline \text { B. } & \text { Saccharomyces cerevisiae } & \text { II. } & \text { Streptokinase } \\
\hline \text { c. } & \text { Trichoderma polysporum } & \text { III. } & \text { Butyric acid } \\
\hline \text { D. } & \text { Streptococcus sp. } & \text { IV. } & \text { Cyclosporin- } \\
\hline
\end{array}\\
&\text { Choose the correct answer from the options given below: }
\end{aligned}
\)
 [NEET 2024]
\(
\begin{aligned}
&\text { Answer (3) }\\
&\begin{array}{lll}
\text { Sol. A. Clostridium butylicum } & – & \text { Butyric acid } \\
\text { B. Saccharomyces cerevisiae } & – & \text { Ethanol } \\
\text { C. Trichoderma polysporum } & – & \text { Cyclosporin-A } \\
\text { D. Streptococcus } s p . & – & \text { Streptokinase }
\end{array}
\end{aligned}
\)
Which one of the following statements is NOT correct? [NEET 2023]
Answer (2)
Sol. Algal bloom imparts a distinct colour to the water bodies. It causes deterioration of the water quantity and fish mortality.
Select the mismatch. (NEET 2017)
(a) : Rhodospirillum is a free-living nitrogen fixing bacteria. Mycorrhiza is the symbiotic association between fungi and roots of higher plants. The most common fungal partners of mycorrhiza are Glomus species.
Which of the following in sewage treatment removes suspended solids? (NEET 2017)
(b) : Primary or physical treatment is the process of removal of small and large, floating and suspended solids from sewage through two processes of filtration and sedimentation.
Which of the following is correctly matched for the product produced by them? (NEET 2017)
(c) : Methanobacterium is useful in the production of biogas. Penicillium notatum is used to produce penicillin, an antibiotic. Acetobacter aceti is used to obtain acetic acid.
(b)
(b) : Clostridium butylicum helps in the production of butyric acid. Candida lipolytica and Geotrichum candidum help in production of lipases that are added in detergents for removing oily stains from laundry.
(c)
The guts of cow and buffalo possess (2015 Cancelled)
(a) : Methanogens like Methanobacterium are found in the rumen (a part of the stomach) of cattle. A lot of cellulosic material is also available in the rumen. In rumen, these bacteria help in the breakdown of cellulose and play an important role in nutrition of cattle.
Which one of the following fungi contains hallucinogens? (NEET 2014)
(b) : Amanita muscaria is a member of Class Basidiomycetes. It is a poisonous mushroom and has hallucinogenic properties. It produces a toxic alkaloid, muscarine, which mimics the effects of acetylcholine and binds to muscarinic receptors as well as ibotenic acid which also binds to different receptors. This leads to excitation of neurons bearing these receptors and hence hallucinations.
An alga which can be employed as food for human being is (NEET 2014)
(b) : Chlorella is a single celled chlorophycean alga with rich protein content. It is considered as a source of SCP (single cell protein) and also as food source during long space travels.
What gases are produced in anaerobic sludge digesters? (NEET 2014)
(b) : The type of gases produced by microbial activity depend upon the microbes and the organic substrates they utilise. Certain bacteria, called methanogens, grow anaerobically on cellulosic material and produce large amount of methane along with carbon dioxide. These bacteria are commonly found in the anaerobic sludge during sewage treatment. Other anaerobic bacteria, involved in the process of anaerobic digestion produce other gases like ammonia and hydrogen sulphide.
A good producer of citric acid is (NEET 2013)
(c) : Aspergillus niger produces citric acid, Clostridium butylicum produces butyric acid,Saccharomyces is used for commercial production of ethanol, Pseudomonas produces alkaline proteases.
Microbe used for biocontrol of pest butterfly caterpillars is (Karnataka NEET 2013)
(b) : Biopesticides are those biological agents that are used for control of weeds, insects and pathogens. The microorganisms used as biopesticides are viruses, bacteria, protozoa, fungi and mites. Some of the biopesticides are being used at a commercial scale. Most important example is the soil bacterium, Bacillus thuringiensis \((\mathrm{Bt})\). Spores of this bacterium produce the insecticidal cry protein. Therefore, spores of this bacterium kill larvae of insects which eat them.
Monascus purpureus is a yeast used commercially in the production of (NEET 2012)
(d) : Statins are produced by the yeast Monascus purpureus which have been commercialised as bloodcholesterol lowering agents. It acts by competitively inhibiting the enzyme responsible for synthesis of cholesterol.
A patient brought to a hospital with myocardial infarction is normally immediately given (NEET 2012)
(b) : Streptokinase (Tissue Plasminogen Activator or TPA) is an enzyme obtained from the culture of some haemolytic bacterium Streptococcus which is modified genetically to function as clot buster. Therefore, it helps in clearing blood clots inside the blood vessels through dissolution of intravascular fibrin during myocardial infarction.
A nitrogen-fixing microbe associated with Azolla in rice fields is (NEET 2012)
(b) : Azolla plays a very important role in rice production. Azolla and its nitrogen-fixing partner, Anabaena, have been used as green manure to fertilise rice paddies and increase production. With the help of Azolla, rice can be grown year after year, several crops a year, with little or no decline in productivity; hence no rotation of crops is necessary.
Which one of the following is an example of carrying out biological control of pests/diseases using microbes?
(NEET 2012)
(a) : A biological control being developed for use in the treatment of plant disease is the fungus Trichoderma. Trichoderma species are free living fungi that are very common in the root ecosystems. They are effective biocontrol agents of several plant pathogens.
In gobar gas, the maximum amount is that of (NEET 2012)
(b) : Biogas or gobar gas is a methane rich fuel gas produced by anaerobic breakdown or digestion of biomass with the help of methanogenic bacteria. It is made up of methane ( \(50-70 \%\) ), carbon dioxide (30 – \(40 \%\) ) with traces of nitrogen, hydrogen sulphide and hydrogen.Â
Read the following four statements (A-D).
(A) Colostrum is recommended for the new born because it is rich in antigens.
(B) Chikungunya is caused by a Gram negative bacterium.
(C) Tissue culture has proved useful in obtaining virus-free plants.
(D) Beer is manufactured by distillation of fermented grape juice.
How many of the above statements are wrong? (NEET 2012)
(b) : Chikungunya is caused by Chikungunya virus. Colostrum is the first breast milk of mother which contains antibodies (especially \(\operatorname{Ig} \mathrm{A}\) ) which protect the infant by the age of three months. Beer is manufactured by fermentation of barley malt by yeast species.
The domestic sewage in large cities (Mains 2012)
(b) : Sewage water can be purified by passing it through sewage treatment plants with the action of heterotrophic microorganisms. There are three stages of this treatment – primary, secondary and tertiary. Primary treatment removes floating and suspended solids from sewage through two processes of filtration and sedimentation. First floating matter is removed through sequential filtration. The filtrate is kept in large open settling tanks where grit settles down. The sediment is called primary sludge while the supernatant is called effluent. The primary sludge traps a lot of microbes and debris. In secondary treatment, the primary effluent is taken to aeration tanks. A large number of aerobic heterotrophicmicrobes grow in the aeration tank. They form flocs. Flocs are masses of bacteria held together by slime and fungal filaments to form mesh like structures. The microbes digest a lot of organic matter, converting it into microbial biomass and releasing a lot of minerals. As the BOD of the waste matter is reduced to \(10-15 \%\) of raw sewage, it is passed into settling tank. Thus secondary treatment is more or less biological. The sediment of settling tank is called activated sludge. The remaining is passed into a large tank called anaerobic sludge digester. It is designed for continuous operation. The aerobic microbes present in the sludge get killed. Anaerobic microbes digest the organic mass as well as aerobic microbes of the sludge. They are of two types, nonmethanogenic and methanogenic. Methanogenic bacteria produce a mixture of gases containing methane, \(\mathrm{H}_2 \mathrm{~S}\) and \(\mathrm{CO}_2\).Â
Consider the following four statements \((\mathrm{A}-\mathrm{D}\) ) and select the option which includes all the correct ones only.
(A) Single cell Spirulina can produce large quantities of food rich in protein, minerals, vitamins, etc.
(B) Body weight-wise the microorganism Methylophilus methylotrophus may be able to produce several times more proteins than the cows per day.
(C) Common button mushrooms are a very rich source of vitamin C.
(D) A rice variety has been developed which is very rich in calcium. (Mains 2012)
(d)
The most common substrate used in distilleries for the production of ethanol is (NEET 2011)
(d)
Which one of the following is not a biofertiliser? (NEET 2011)
(a) : Biofertilizers are organisms that enrich the nutrient quality of the soil. The main sources of biofertilizers are bacteria, fungi and cyanobacteria. Rhizobium bacteria is found in the nodules on the roots of leguminous plants by symbiotic association. These bacteria fix atmospheric nitrogen into organic forms, which is used by the plants as nutrient. Fungi are also known to form symbiotic associations with plants called mycorrhiza. Cyanobacteria are autotrophic microbes widely distributed in an aquatic and terrestrial environments. Many of which can fix atmospheric nitrogen, e.g., Anabaena, Nostoc, Oscillatoria etc. But Agrobacterium tumefaciens is a pathogen of several dicot plants. It causes gall tumor in the plants.
Secondary sewage treatment is mainly a (NEET 2011)
(d) \(\square\)
Which of the following is mainly produced by the activity of anaerobic bacteria on sewage? (NEET 2011)
(d) : Marsh gas or methane gas is mainly produced by the activities of anaerobic bacteria on sewage. Sewage contains large amounts of organic matter and microbes, many of which are pathogenic. These microbes (bacteria and fungi) are digested during secondary treatment process of sewage by anaerobic bacteria. During digestion, bacteria produce a mixture of gases such as methane, hydrogen sulphide and carbon dioxide. These gases are called biogas and can be easily used as a source of energy as it is inflammable.
An organism used as a biofertilizer for raising soyabean crop is (NEET 2011)
(c) : Rhizobium is used as a biofertilizer for raising crop. Rhizobium japonicum forms symbiotic association in the roots of the leguminous plant, soyabean.
Ethanol is commercially produced through a particular species of (NEET 2011)
(a) : Ethanol is commercially produced through a particular species of yeast Saccharomyces (Saccharomyces cerevisiae).
Continuous addition of sugars in ‘fed batch’ fermentation is done to (NEET 2011)
(b)
Organisms called methanogens are most abundant in a (NEET 2011)
(b) : Methanogens are any of various archaebacteria that produce methane; they include genera such as Methanobacillus and Methanothrix. Methanogens are obligate anaerobes found in oxygendeficient environments, such as marshes, swamps, sludge and the digestive systems of ruminants. They are also utilised in gobar gas plants.
Consider the following statements \(\)(A-D)\(\) about organic farming.
(A) Utilizes genetically modified crops like Bt cotton
(B) Uses only naturally produced inputs like compost
(C) Does not use pesticides and urea
(D) Produces vegetables rich in vitamins and minerals
Which of the above statements are correct? (NEET 2011)
(c) : Organic farming is the form of agriculture that relies on techniques such as crop rotation, green manure, compost and biological pest control to maintain soil productivity and control pest on a farm. Organic farming excludes or strictly limited the use of manufactured fertilizers, pesticides (which include herbicides, insecticides and fungicides), plant growth regulators such as hormones, food additives and genetically modified organisms.
Which one of the following is a wrong matching of a microbe and its industrial product, while the remaining three are correct?
(Mains 2011)
(c) : Microbes are used for commercial and industrial production of certain chemicals like organic acids, alcohols and enzymes. Examples of acid producers are Aspergillus niger (a fungus) of citric acid, Acetobacter aceti (a bacterium) of acetic acid; Clostridium butylicum (a bacterium) of butyric acid and Lactobacillus (a bacterium) of lactic acid.
Select the correct statement from the following. (NEET 2010)
(d):
A common biocontrol agent for the control of plant diseases is (NEET 2010)
(d) : The natural method of pest and pathogen control involving use of viruses, bacteria and other insects (which are their natural predators and pests) is called biocontrol or biological control. For example, free living fungus Trichoderma exerts biocontrol over several plant pathogens for the control of plant diseases. Baculoviruses (mostly of genus Nucleopolyhedrovirus) are also used as biocontrol agents but they are used for the control of insects and arthropods. Bacillus thuringiensis is a soil bacterium which is used as biopesticide. Glomus species are the most common fungal partners of mycorrhiza residing in the roots of higher plants.
The common nitrogen-fixer in paddy fields is (NEET 2010)
(b) : Azospirillum is an anaerobic nitrogen fixing bacteria which forms loose association with roots of some plants. Inoculation of paddy fields with these bacteria helps in increasing yield and saving of nitrogen fertilizers.
Which one of the following is not used in organic farming? (NEET 2010)
(d) : Organic farming is a method of farming system which primarily aimed to keep the soil alive and in good health by use of organic wastes and other biological material alongwith beneficial microbes (biofertilizers) to release nutrients to crops for increased sustainable production in an ecofriendly, pollution free environment.
Basic components of organic farming are green manures, farm yard manure, vermicompost, crop rotation, biopesticides and biofertilizers. Glomus being a mycorrhizal component, earthworm being a vermicompost and Oscillatoria being a nitrogen fixing blue green algae can be used in organic farming. Snail cannot be a component of organic farming.
An example of endomycorrhiza is (Mains 2010)
(b) : The genus Glomus form endomycorrhiza, a symbiotic associations with plants. The fungal symbiont in these associations absorbs phosphorus from soil and passes it to the plant. Plants having such associations show other benefits also, such as resistance to root-borne pathogens, tolerance to salinity and drought, and an overall increase in plant growth and development.
Nostoc is a blue green algae, Agaricus is a basidiomycetes, Rhizobium is a eubacteria.
Which one of the following pairs is wrongly matched? (NEET 2009)
(a): Yeast species are used in alcoholic fermentation due to the presence of zymase enzyme. It was known that the yeast extract contained an enzyme zymase, which is nondialyzable and a coenzyme which is dialyzable. It is now well known that the zymase is a complex mixture of many enzymes and that several coezymes are necessary for their function. The activity of this enzyme was lost because the main enzyme was separated from its coezyme during dialyzation. Thus, it was established later that extracellular enzyme zymase, secreted by yeast cells, carry out the process of fermentation.
Which of the following is not used as a biopesticide? (NEET 2009)
(c) : The natural method of pest and pathogen control involving use of viruses, bacteria and other insects (which are their natural predators and pests) is called biocontrol or biological control. For example, free living fungus Trichoderma exerts biocontrol over several plant pathogens for the control of plant diseases. Baculoviruses (mostly of genus Nucleopolyhedrovirus) are also used as biocontrol agents but they are used for the control of insects and arthropods. Bacillus thuringiensis is a soil bacterium which is used as biopesticide. Glomus species are the most common fungal partners of mycorrhiza residing in the roots of higher plants.Â
Trichoderma harzianum has proved a useful microorganism for (NEET 2008)
(b) : The natural method of pest and pathogen control involving use of viruses, bacteria and other insects (which are their natural predators and pests) is called biocontrol or biological control. For example, free living fungus Trichoderma exerts biocontrol over several plant pathogens for the control of plant diseases. Baculoviruses (mostly of genus Nucleopolyhedrovirus) are also used as biocontrol agents but they are used for the control of insects and arthropods. Bacillus thuringiensis is a soil bacterium which is used as biopesticide. Glomus species are the most common fungal partners of mycorrhiza residing in the roots of higher plants.Â
Nitrogen fixation in root nodules of Almus is brought about by (NEET 2008)
(a): The most common symbiotic association of legume and bacteria on roots is as nodules, which are small outgrowth on the roots. The microbe Frankia is symbiont in root nodules of several non-legume plants like Casurina and Alnus. Both Rhizobium and Frankia are free living in soil but as symbiont can fix atmospheric nitrogen.
Which one of the following proved effective for biological control of nematodal diseases in plants? (NEET 2008)
(b) : Paecilomyces lilacinus proved effective for biological control of nematodal diseases in plant.
Probiotics are (NEET 2007)
(c) : Probiotics are dietary supplements containing potentially beneficial bacteria or yeast, with lactic acid bacteria (LAB) as the most commonmicrobes used. LAB have been used in the food industry for many years, because they are able to convert sugars (including lactose) and other carbohydrates into lactic acid. They not only provides the characteristic sour taste of fermented dairy foods such as yogurt, but acts as a preservative, by lowering the \(\mathrm{pH}\) and creating fewer opportunities for spoilage organisms to grow.
Which one of the following pairs is wrongly matched? (NEET 2007)
(c) : Coliform bacteria are a commonly used bacterial indicator of sanitary quality of foods and water. It is not involved in the production of vinegar.
Dough kept overnight in warm weather becomes soft and spongy because of (NEET 2004)
(b)
The most thoroughly studied of the known bacteria-plant interactions is the (NEET 2004)
(b): Agrobacterium tumefaciens is the causative agent of crown gall, an important disease of many commercial crops. This disease has come to be recognized in recent years as being caused by a DNA plasmid (Ti plasmid) carried by bacterium and transferred to the plant cells.
A major component of gobar gas is (NEET 2004)
(b) :
During anaerobic digestion of organic waste, such as in producing biogas, which one of the following is left undegraded?
(NEET 2003)
(b) : Lignin is a complex polymer of phenylpropane units, which are cross-linked to each other with a variety of different chemical bonds. This complexity has thus far proven as resistant to microbial degradation.
Which bacteria is utilized in gobar gas plant? (NEET 2002)
(a):
During the formation of bread it becomes porous due to release of \(\mathrm{CO}_2\) by the action of (NEET 2002)
(a): Saccharomyces converts starch or sugars to pyruvic acid through EMP pathway. Then this pyruvic acid is converted to acetaldehyde and finally to ethyl alcohol in the absence of oxygen. This entire process is called fermentation.
Which of the following is the pair of biofertilizers? (AIPMT 2001)
(a)
Spoilage of oil can be detected by which fatty acid (AIPMT 2001)
(d) : Erucic acid is an unsaturated fatty acid belonging to the oleic acid series, occurring as glycerides in rape seed oil and other vegetable oils. It is the cis-isomer, the trans-isomer being brassidic acid. Erucic acid is used as a binder for oil paints. It is useful in manufacture of emulsions to coat photographic films and papers. Oleic acid is found in various animal and vegetable sources. It is widely used in industries including textile, chemical, medicine, leather, stationary, paper making, etc. Linolenic acids is used in making soaps, emulsifiers and quick-dryingoils, in beauty products. It helps in acne reduction, moisture retention, etc. Linoleic aicd is an important fatty acid especially for growth and development of infants. Commercially it is used in margarine, animal feeds, soaps and drugs.
Which aquatic fern is used to increase the yield in paddy crop? (AIPMT 2000)
(a):
Which of the following is used to manufacture ethanol from starch? (AIPMT 2000)
(b) : Saccharomyces converts starch or sugars to pyruvic acid through EMP pathway. Then this pyruvic acid is converted to acetaldehyde and finally to ethyl alcohol in the absence of oxygen. This entire process is called fermentation.Â
Which of the following fern is an excellent biofertilizer? (AIPMT 1999)
(c):
(d) : Biofertilizers are organisms which bring about nutrient enrichment of the soil. Azotobacter is a free living, aerobic, nitrogen fixing bacteria. Anabena is a nitrogen fixing cyanobacteria that occurs in both free living and symbiotc associations with Azolla, Cycas roots, etc. Rhizobium lives symbiotically in root nodules of legumes and nonlegumes. Vesicular-arbuscular mycorrhiza (VAM) is an example of endomycorrhiza in which fungal hyphae penetrate the cortical cells of grasses to form vesicles.
Yeast (Saccharomyces cerevisiae) is used in the industrial production of (AIPMT 1998)
(b) : Saccharomyces converts starch or sugars to pyruvic acid through EMP pathway. Then this pyruvic acid is converted to acetaldehyde and finally to ethyl alcohol in the absence of oxygen. This entire process is called fermentation.
Farmers have reported over \(50 \%\) higher yields of rice by using which of the following biofertilizer?
(AIPMT 1998)
(d) :
Which of the following microorganisms is used for production of citric acid in industries? (AIPMT 1998)
(a) : Citric acid is obtained through the fermentation carried out by Aspergillus niger on sugary syrups. Citric acid is employed in dyeing, engraving, medicines, inks, flavouring and preservation of food and candies.
Biological control component is central to advanced agricultural production. Which of the following is used as a third generation pesticide?
(AIPMT 1998)
(d) : Pheromones are volatile chemicals produced by a given species to communicate with other individuals of the same species to change their behaviour. The synthetic versions of lepidopteran pheromones can be used as pesticides. Sometimes the relative amount of several pheromone chemicals in a pesticide product determine which specific pests are controlled when the pesticide product releases pheromone into the air where males are looking for females, the males become confused and cannot easily locate the females. As a result, many of the females do not mate and lay eggs and there are many fewer offspring than usual. These insect pheromones are third generation pesticides. Other third generation pesticides are insect growth regulators, chitin
The biofertilizers are (AIPMT 1997)
(a):
Gobar gas contains mainly (AIPMT 1997)
(d) :
Cochineal insects have proved very useful for (AIPMT 1996)
(a) : Extensive growth of Opuntia (Cactus) in Australia was checked through introduction of its natural herbivore, cochineal insect (Cactoblastis cactorum).
When a natural predator (living being) is applied on the other pathogen organism to control them. this process is called
(AIPMT 1996)
(c) : The control of insect pests by the introduction, encouragment and artificial increase of biological agencies like predaceous and parasitic insects, other animals and diseases is termed as biological control. It is basically a natural control in which man plays significant role of making the biological agencies more effective. Of these agencies, insect enemies play important role in nature for managing the phytophagous insect pests and keep a balance e.g., lady bugs or praying mantis, frog, toads, lizard and birds are employed by man to eat up the insect pests like aphids. It is a self perpetuating method.
The citric acid is produced by (AIPMT 1995)
(c) :
The rotenone is (AIPMT 1995)
(b) : Natural insecticides are those which are obtained from microorganisms and plants. The first natural insecticide used by man is azadirachtin obtained from Azadirachta indica.
Rotenone is another natural insecticide which is obtained from the roots of Derris and Lonchocarpers. It is harmless to warm blooded animals.
The organism, used for alcohol fermentation, is (AIPMT 1995)
(b)Â : Â Saccharomyces converts starch or sugars to pyruvic acid through EMP pathway. Then this pyruvic acid is converted to acetaldehyde and finally to ethyl alcohol in the absence of oxygen. This entire process is called fermentation.
One of the major difficulties in the biological control of insect pest is that (AIPMT 1995)
(d) : Insect enemies play important role in nature for managing the phytophagous insect pests and keep a balance. It is just possible that predators of a particular plant pest are unable to get established and multiply in a particular environment. In such cases, the predators are reared in the laboratory and let off at a particular time when the pests are about to threaten the crops. And then this practice become expensive.
Biological control of agricultural pests, unlike chemical control, is (AIPMT 1994)
(a):
In cheese manufacture, the microorganisms are important for (AIPMT 1994)
(d) : Cheese is a protein rich nutritive preparation obtained after fermentation and curding of milk. Cheese contains proteins \((20-35 \%)\), fats \((20-30 \%)\), minerals, vitamins and water. Milk is first curdled (soured) with the help of a lactic acid bacterium. Curd is gently heated to separate cheese from liquid called whey. Curd is placed in cloth-lined porous containers for draining out whey. The left out solidified material is called cottage cheese. For preservation and ripening, blocks of cottage cheese are salted and placed in brine solution. Salt solution is drained out. Cheese blocks are wiped and placed in sterilised rooms for ripening with the help of microorganisms.
Which of the following species does not have the ability to fix atmospheric nitrogen? (AIPMT 1994)
(d) : Members of Kingdom Monera – bacteria and cyanobacteria (blue green algae) have the ability to fix nitrogen. Azotobacter is a \(\mathrm{N}_2\)-fixing bacteria. Anabaena and Nostoc are heterocystous blue-green algae. The heterocysts are the sites of \(\mathrm{N}_2\)-fixation. Spirogyra is one of the commonest green algae. It has no function in nitrogen fixation.
Which one of the following statements is correct? (AIPMT 1994)
(a) : The nitrogen-fixing ability of leguminous plants is not a property of the plants as such but results from infection of their roots by bacteria in the soil, infection leading to the formation of nodules. These organisms are Gram-negative motile rods that are classified in the genus Rhizobium.
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